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Optimization of a Quantitative Micro-neutralization Assay
Published on: December 14, 2016
Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization
Annette A Kraus1, William Messer, Laura B Haymore
1Department of Microbiology and Immunology, CB#7290, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
Journal of Clinical Microbiology
|September 7, 2007
Summary
New flow-based assays offer rapid, quantitative measurement of dengue neutralization. These high-throughput assays use human cells, improving upon traditional methods as dengue vaccines advance.
Area of Science:
- Virology
- Immunology
- Vaccinology
Background:
- Dengue virus (DENV) poses a significant global health threat.
- The development of effective dengue vaccines is ongoing, with several candidates in clinical trials.
- Accurate and efficient methods to measure vaccine-induced neutralization are crucial for vaccine development.
Purpose of the Study:
- To develop and validate rapid, quantitative, and high-throughput neutralization assays for dengue virus.
- To compare the performance of novel flow-based assays with the established plaque reduction neutralization test (PRNT).
Main Methods:
- Development of flow cytometry-based neutralization assays utilizing human cells.
- Comparison of results from flow-based assays with those from the plaque reduction neutralization test (PRNT).
Main Results:
- Flow-based neutralization assays demonstrated comparable results to the established PRNT.
- The developed flow assays enable high-throughput screening.
- The assays utilize human cells, offering a more relevant model.
Conclusions:
- Flow-based neutralization assays represent a significant advancement for evaluating dengue vaccines.
- These assays provide a rapid, quantitative, and high-throughput alternative to traditional methods.
- The use of human cells enhances the relevance of these assays for vaccine immunogenicity studies.
