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Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
Published on: March 18, 2011
Glyceryl ethers in insects: identification of alkyl and alk-1-enyl glyceryl ether phospholipids
1Medical Division, Lipid Research Laboratory, Oak Ridge Associated Universities, 37830, Oak Ridge, Tennessee.
Abstract:
Alkyl and alk-1-enyl glyceryl ethers have been identified in the phospholipids of three insect species, the American cockroach (Periplaneta americana), the tobacco budworm (Heliothis virescens), and the boll weevil (Anthonomus grandis). Glyceryl ethers were not detected in the neutral lipids. The ethers were found in the phospholipid fraction of whole insects and in isolated fat body tissue. The ether content varied among the three insect species, and fluctuated during various developmental stages. Gas liquid chromatographic analysis of the alkyl glyceryl ethers and aldehydes derived from the alk-1-enyl glyceryl ethers of the cockroach and budworm whowed striking differences in chain length. However, the hydrocarbon side-chain of the two ether fractions were similar in length for each species. Preliminary evidence indicates that 1-(14)C-acetate can be incorporated into alkyl ethers but not into alk-1-enyl ethers ofHeliothis pupae.
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