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Updated: Jul 12, 2026

Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal
Published on: September 10, 2021
Tritium and(14)C counting in tissue samples by using liquid scintillation method
1Colgate Palmolive Company, 08854, Piscataway, New Jersey.
Abstract:
The combustion method has been modified to increase the recovery of tritiated water after combustion of a tritium-labeled tissue sample. This was accomplished by cooling the bottom of the combustion flask in a dry ice-acetone bath while irradiating the top with an infrared lamp. The procedure resulted in at least 92% to 102% recovery of the tritiated water. The NCS solubilizer was found to be superior to hyamine for solubilizing(14)C labeled tissue samples. The samples yielded light yellow-colored solutions when incubated for 15 hr at 50-55C. The counting efficiency of this solution was 75% or higher.
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