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Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Related Experiment Video

Updated: Jul 12, 2026

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)
09:58

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Crustacean eye fine structure seen with scanning electron microscopy.

T H Waterman, A S Pooley

    Science (New York, N.Y.)
    |July 11, 1980
    PubMed
    Summary

    Scanning electron microscopy revealed the detailed internal structure of crustacean compound eyes. This study offers new insights into the functional organization of photoreceptive, dioptric, and screening components in various species.

    Area of Science:

    • Zoology
    • Microscopy
    • Ophthalmology

    Background:

    • Crustacean compound eyes possess complex structures for vision.
    • Previous understanding of their fine internal organization was limited.

    Purpose of the Study:

    • To reexamine the internal fine structure of crustacean compound eyes.
    • To gain new insights into the functional organization of eye components.

    Main Methods:

    • Comparative study using scanning electron microscopy.
    • Application of several different preparative techniques.
    • Examination of eyes from crabs, crayfish, shrimp, and stomatopods.

    Main Results:

    • Direct demonstration of the three-dimensional pattern of photoreceptive, dioptric, and screening components.

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  • Detailed visualization of boundary membranes and protoplasmic strands in apposition eyes.
  • Insight into intracellular structures linking microvilli to cytoplasm.

    • Conclusions:

    • Scanning electron microscopy provides advanced understanding of crustacean eye functional organization.
    • The findings highlight the intricate cellular architecture supporting visual processing.
    • Further quantitative analysis can advance understanding of receptor membrane turnover.