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Updated: Jul 12, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
Relation between the Inorganic Chemistry and Biochemistry of Bone Mineralization
Abstract:
In vitro experiments with saliva resulted in precipitation of a mineral substance (dahllite or carbonate hydroxyapatite) which is comparable in composition and crystal structure to oral calculus. Similar mineral substances were produced from synthetic solutions containing sodium phosphate and calcium chloride (in addition to a buffer) in the presence of carbonic anhydrase and available carbon dioxide. It is concluded that the carbonate ion is essential to precipitation of bone mineral and that the principal biochemical catalyst in vivo is carbonic anhydrase. Bacteria are not essential to the precipitation, but they probably play a secondary role in connection with the formation of oral calculus, urinary calculus, and so forth.
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