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Related Experiment Videos

Shear effects on suspended cells.

J C Merchuk1

  • 1Department of Chemical Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel.

Advances in Biochemical Engineering/Biotechnology
|January 1, 1991
PubMed
Summary

This study explores how shear forces affect cell cultures before damage occurs. Understanding these non-damaging shear effects is crucial for optimizing bioreactor design and scale-up.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Cell Culture Technology

Background:

  • Shear forces are typically viewed as destructive to microorganisms and cells in technical literature.
  • Existing research primarily focuses on identifying shear levels that cause damage to cell cultures.

Purpose of the Study:

  • To investigate the effects of non-damaging shear forces on suspended cell cultures.
  • To review and critically analyze devices used for studying shear effects on cultures.

Main Methods:

  • Literature review of shear effects on cell cultures.
  • Critical evaluation of available devices for shear studies.
  • Analysis of shear influence on cellular processes.

Main Results:

  • Shear forces, below damaging levels, can influence growth rate, cellular volume, and metabolite production.
  • Liquid dynamics within bioreactors can impact the kinetics of biochemical processes.
  • Available shear study devices were reviewed and assessed.

Conclusions:

  • Non-damaging shear forces have significant impacts on cell culture parameters.
  • Hydrodynamics play a critical role in bioreactor performance.
  • Structural bioreactor models are essential for accurately describing system hydrodynamics and optimizing bioreactor design and scale-up.

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