Monkey photoreceptor calycal processes and interphotoreceptor matrix as observed by scanning electron microscopy

Photoreceptors and the interphotoreceptor matrix of monkey retina were observed by scanning electron microscopy. Cone photoreceptors are easily distinguished from rod photoreceptors by their wide conical inner segments. The calycal processes of 50 rods and 50 cones were counted and measured. The calycal processes of cones were distinct, short, and uniform in diameter (0.1 micron). They were arranged equidistantly and, in most cases, were not continuous with longitudinal inner segment ridges, as previously suggested. In contrast to cones, rod calycal processes were fewer in number, were about one-fourth the number of the cones, were of variable length (0.7 micron to 3.0 micron), and tapered to a fine point at their distal ends. The interphotoreceptor matrix appeared spongelike, made up of anastomosing plates and strands filling the interphotoreceptor space. Other than an increased amount of matrix around cones, no structural difference between rod- and cone-associated interphotoreceptor matrix was observed.