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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Related Experiment Video

Updated: Jul 12, 2026

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

Computed tomographic analysis of meteorite inclusions.

J R Arnold, J P Testa, P J Friedman

    Science (New York, N.Y.)
    |January 28, 1983
    PubMed
    Summary

    Computed tomography non-destructively probes the Allende meteorite's interior, revealing high and low atomic number regions. This allows targeted analysis of calcium- and aluminum-rich inclusions, advancing solar system origin studies.

    Area of Science:

    • Cosmochemistry
    • Planetary Science
    • Geophysics

    Background:

    • Isotopic anomalies in calcium- and aluminum-rich inclusions (CAIs) of the Allende meteorite offer insights into solar system origins.
    • Previous studies were limited by the destructive nature of meteorite sample retrieval.
    • A need existed for non-destructive methods to analyze heterogeneous meteorite interiors.

    Purpose of the Study:

    • To develop and apply a non-destructive method for analyzing the interior of the Allende meteorite.
    • To identify and isolate specific regions of interest within the meteorite for further isotopic analysis.
    • To overcome limitations of previous destructive sampling techniques.

    Main Methods:

    • Utilized a fourth-generation computed tomographic (CT) scanner with software modifications.

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    Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction
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    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
    10:12

    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

    Published on: June 19, 2018

    Related Experiment Videos

    Last Updated: Jul 12, 2026

    Atom Probe Tomography Analysis of Exsolved Mineral Phases
    08:14

    Atom Probe Tomography Analysis of Exsolved Mineral Phases

    Published on: October 25, 2019

    Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction
    09:13

    Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction

    Published on: April 1, 2017

    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
    10:12

    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

    Published on: June 19, 2018

  • Applied CT scanning to nondestructively probe the heterogeneous interior of the Allende meteorite.
  • Developed image processing to differentiate regions based on atomic number.
  • Main Results:

    • Successfully visualized the interior structure of the Allende meteorite.
    • Distinguished regions with high and low atomic numbers rapidly.
    • Demonstrated the capability to precisely target and isolate specific inclusions for analysis.

    Conclusions:

    • Computed tomography provides an effective non-destructive means to study meteorite interiors.
    • This technique facilitates the targeted retrieval of scientifically valuable samples, such as CAIs.
    • Advancements in CT scanning enhance the study of solar system formation and evolution.