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Related Experiment Video

Updated: Jul 12, 2026

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

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Published on: August 13, 2011

Cellulases Can Enhance beta-Glucan Synthesis.

Y S Wong, G B Fincher, G A Maclachlan

Science (New York, N.Y.)

|February 18, 1977

Summary

Preliminary treatment with purified cellulases significantly boosts beta-glucan synthesis in pea plants. This suggests cellulases act as primers, enhancing cellulose microfibril chain elongation for improved plant cell wall development.

Area of Science:

Plant biochemistry
Cell wall biosynthesis

Background:

Beta-glucan is a crucial component of plant cell walls.
Cellulose microfibrils provide structural integrity to plant tissues.

Purpose of the Study:

To investigate the effect of cellulase treatment on beta-glucan synthesis.
To explore the mechanism by which cellulases influence polysaccharide production.

Main Methods:

Pea epicotyl tissue slices were treated with purified cellulases.
Beta-glucan synthesis was quantified using uridine diphosphoglucose as a substrate.

Main Results:

Cellulase treatment increased beta-glucan synthesis by two- to threefold.
The effect was observed after short-term, preliminary treatment.

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Conclusions:

Cellulases may introduce chain ends in cellulose microfibrils.
These chain ends likely serve as primers for beta-glucan chain elongation.