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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Three-Dimensional Microscopy in Microbiology

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Computed Tomography

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X-ray Imaging

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Related Experiment Video

Updated: Jul 12, 2026

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Three-Dimensional X-ray Microtomography.

B P Flannery, H W Deckman, W G Roberge

    Science (New York, N.Y.)
    |September 18, 1987
    PubMed
    Summary

    X-ray microtomography offers a new non-destructive method to create detailed 3D maps of material composition. This technique achieves high accuracy and resolution, enabling precise elemental analysis for small samples.

    Area of Science:

    • Materials Science
    • Physics
    • Imaging Technology

    Background:

    • X-ray microtomography is a powerful technique for non-destructive analysis.
    • Accurate 3D mapping of material properties is crucial in various scientific fields.

    Purpose of the Study:

    • To introduce and describe a new x-ray microtomography technique.
    • To demonstrate its capability for generating high-accuracy, high-resolution 3D maps.
    • To showcase elemental mapping using synchrotron sources.

    Main Methods:

    • Utilized x-ray microtomography for non-destructive 3D imaging.
    • Employed synchrotron x-ray sources for spatially resolved elemental mapping.
    • Developed high-speed algorithms for tomographic reconstruction.
    • Integrated a high-resolution imaging x-ray detector.

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    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
    08:51

    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

    Published on: May 27, 2008

    Related Experiment Videos

    Last Updated: Jul 12, 2026

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
    08:46

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

    Published on: April 13, 2016

    High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
    08:57

    High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

    Published on: June 21, 2011

    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
    08:51

    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

    Published on: May 27, 2008

    Main Results:

    • Achieved approximately 1 percent accuracy in x-ray attenuation coefficient mapping.
    • Reached a spatial resolution approaching 1 micrometer.
    • Successfully produced spatially resolved elemental maps.
    • Presented tomographic images demonstrating performance with synchrotron and laboratory sources.

    Conclusions:

    • The new x-ray microtomography technique provides accurate and high-resolution 3D material characterization.
    • It enables detailed elemental analysis, particularly with synchrotron radiation.
    • The system is effective for both synchrotron and laboratory-based applications.