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Quantification of Y chromosome bearing spermatozoa of cattle using in situ hybridization
M Schwerin1, S Blottner, P D Thomsen
1Department of Molecular Genetics, Research Center of Animal Production Dummerstorf-Rostock, Federal Republic of Germany.
Molecular Reproduction and Development
|September 1, 1991
Abstract:
An easy assay for quantification of Y chromosome-bearing sperm (Y-sperm) is needed, especially to monitor sperm separation techniques. In the present study a tritiated bovine male-specific DNA fragment was tested for identification of Y-sperm by in situ hybridization. A protocol for in situ hybridization to bovine sperm was developed and used to study the proportion of Y-sperm of 12 bulls. The usefulness of the method in optimization of sperm separation procedures is illustrated through analysis of fractions of sperm separated by Percoll density gradient centrifugation.