Related Experiment Video
Updated: Jul 11, 2026
07:35
Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection
Published on: June 8, 2020
Analysis of inter-(simple sequence repeat) PCR products from human colorectal cancers
Neng Chen1, Daniel L Stoler, Smitha S Dutt
1Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Oncology Research
|September 8, 2007
Summary
Genomic instability in solid tumors is key to understanding cancer development. Researchers used inter-(simple sequence repeat) PCR to detect genetic alterations in colorectal tumors, confirming its effectiveness in identifying genome changes.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Genomic instability is a hallmark of solid tumors, crucial for understanding tumorigenesis.
- Accurate measurement of genetic alterations is essential for cancer research.
Purpose of the Study:
- To adapt and validate the inter-(simple sequence repeat) PCR (inter-(SSR) PCR) technique for measuring genetic alterations in colorectal tumors.
- To confirm that observed changes in inter-(SSR) PCR products reflect true genomic alterations.
Main Methods:
- Adapted inter-(simple sequence repeat) PCR (inter-(SSR) PCR) to analyze genetic variations in colorectal tumors.
- Cloned and performed BLAT searches on 131 inter-(SSR) PCR sequences.
- Analyzed inter-(SSR) PCR product patterns using gel electrophoresis.
Main Results:
- Inter-(SSR) PCR products were found on almost all chromosomes, indicating broad genomic sampling.
- Changes in inter-(SSR) PCR product patterns on gel electrophoresis correlated with actual genomic alterations.
- The technique effectively detected genetic changes between simple sequence repeats in tumor DNA.
Conclusions:
- Inter-(simple sequence repeat) PCR is a validated method for detecting genomic instability in colorectal cancer.
- This technique provides a reliable way to identify and study genetic alterations during tumorigenesis.
