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Type I interferon regulates pDC maturation and Ly49Q expression.
Makiko Toma-Hirano1, Sahori Namiki, Shoichiro Miyatake
1Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
European Journal of Immunology
|September 8, 2007
Summary
Type I interferon (IFN) inhibits the acquisition of Ly49Q on mouse plasmacytoid dendritic cells (pDCs). This immune signaling molecule also influences pDC development and their distribution within the body.
Area of Science:
- Immunology
- Cell Biology
- Developmental Biology
Background:
- Ly49Q is a molecule expressed on peripheral mouse plasmacytoid dendritic cells (pDCs).
- pDC precursors mature and acquire Ly49Q in the bone marrow before migrating to peripheral tissues.
Purpose of the Study:
- To identify molecules regulating pDC maturation.
- To investigate the role of type I interferon (IFN) in pDC development and Ly49Q expression.
Main Methods:
- In vitro assays to assess Ly49Q acquisition.
- In vivo experiments using poly(I:C) injection in wild-type and IFN-alpha/beta receptor knockout mice.
- Flow cytometry to analyze pDC populations and their differentiation into conventional DC.
Main Results:
- Type I IFN was found to inhibit Ly49Q acquisition on pDCs in vitro.
- Infections inducing type I IFN led to an increased presence of Ly49Q-negative pDCs in bone marrow and lymphoid organs.
- In vivo type I IFN exposure induced the conversion of some Ly49Q-negative pDCs into conventional dendritic cells.
Conclusions:
- Type I IFN plays a regulatory role in pDC development.
- Type I IFN influences the distribution and phenotype of pDCs in vivo.
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