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Updated: Jul 11, 2026
Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
Published on: March 3, 2015
[Screening of polypeptides interacting with Rel homology domain of NF-kappaB p50 subunit]
Li-qun Shen1, Xiang Xu, Hua-ping Liang
1Research Institute of Surgery, Daping Hospital, Chongqing 400042, China.
Aim:
To obtain the polypeptides interacting with NF-kappaB p50 Rel homology domain (RHD) by yeast two-hybrid technique.
Methods:
Using NF-kappaB p50 RHD as bait, the polypeptides interacting with NF-kappaB p50 RHD were screened from a 16-peptide cDNA library by yeast two-hybrid technique. The false positive clones were screened out but the positive clone were identified by beta-GAL assay, yeast mating, and one to one yeast two-hybrid method.
Results:
8 positive clones were obtained. The gene sequences of the eight polypeptides were different from each other and their homologous proteins were not found in GenBank.
Conclusion:
8 novel polypeptides interacting with NF-kappaB p50 RHD were obtained, but their functions need to be validated in further experiments.
