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[Effect of PF4 on 60Co gamma ray irradiation-induced bone marrow cell apoptosis in mice]
Hui-liang Lin1, Lan Yang, Xin-rong Wang
1Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. 314lhl@163.com
Aim:
To study the effect of platelet factor 4 (PF4) on murine bone marrow cell apoptosis after irradiation with 5.0 Gy (60)COgamma and explore its potential mechanism.
Methods:
30 male BALB/c mice were randomly divided into three groups: namely normal control group (n = 6), PF4 treatment group (n=12) and irradiation (IR) group (n = 12). The mice in PF4 treatment group were administrated intraperitoneally PF4 40 microg/kg at 26 h and 20 h respectively before total-body irradiation with 5.0 Gy (60)COgamma ray. At 4 h and 20 h after irradiation the apoptosis of bone marrow cells were detected by flow cytometry (FCM) and the expression of Bax protein was identified by Western blot.
Results:
At 4 h and 20 h after irradiation the apoptosis of bone marrow cells in PF4 treatment group was significantly lower than that in IR group (P < 0.01), the expression of Bax protein in the PF4 group was lower than that in the IR group (P < 0.05).
Conclusion:
PF4 can protect bone marrow cells from radiation-induced apoptosis, which is partly due to the down-regulation of proapoptotic protein Bax expression.
