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Updated: Jul 11, 2026
Generation of Human CD40-activated B cells
Published on: October 16, 2009
[Preparation of a novel functional anti-human CD40 monoclonal antibody and identification of its bioactivity]
Lin Tang1, Lu Zheng, Chun-jian Qi
1Institute of Medical Biotechnology, Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Soochow University, Suzhou 215007, China. tl915@163.com
Aim:
To prepare a novel functional mouse anti-human CD40 monoclonal mAb.
Methods:
Female BALB/c mice of 6-8 weeks old were immunized with CD40 transfectant (L929-CD40) as immunogen. The spleen B cells of the mice were fused with Sp2/0. The hybridoma cells were screened with CD40 transfectant (L929-CD40) by FCM. Fast-strip analysis was performed to identify Ig subclass of this mAb. The epitope recognized by this mAb was detected by Bio/5C11 competitive assay. The proliferation and cell cycle of tumor cells in vitro were studied by MTT assay and PI staining respectively.
Results:
One hybridoma cell line named 2B6 was obtained, which had the property of secreting anti-human CD40 monoclonal antibody continuously and steadily. This mAb specifically recognized human CD40 molecule and promoted the proliferation of tumor cells in vitro.
Conclusion:
One hybridoma cell line which can secret a novel functional mouse anti-human CD40 mAb has been developed successfully. This mAb can specifically recognize human CD40 and influence the growth of tumor cells in vitro.
