Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DISTRIBUTION OF MATURATION-PROMOTING FACTOR IN STARFISH OOCYTE STRATIFIED BY CENTRIFUGATION.

Development, growth & differentiation·2023
Same author

INHIBITION OF GERMINAL VESICLE BREAKDOWN AND POLAR BODY FORMATION BY NICOTINAMIDE IN STARFISH AND SURF CLAM OOCYTES.

Development, growth & differentiation·2023
Same author

Oocyte Competence to Maturation-inducing Hormone. I. Breakdown of Germinal Vesicles of Small Oocytes in Starfish, Asterina pectinifera*: (starfish/oocyte maturation/1-MeAde competence/GVBD/MPF).

Development, growth & differentiation·2023
Same author

Regulation of Metaphase by a Maturation-Promoting Factor: (meiosis/mitosis/cell cycle/metaphase/maturation-promoting factor).

Development, growth & differentiation·2023
Same author

Induction and Inhibition of Meiotic Maturation in Follicle-enclosed Mouse Oocytes by Forskolin: (meiotic maturation/luteinizing hormone/forskolin/cAMP/mouse oocyte).

Development, growth & differentiation·2023
Same author

Inhibition of Starfish Oocyte Maturation by Tumor-Promoting Phorbol Esters*: (starfish oocyte maturation/tumor promoters/phorbol esters/teleocidin/retinoids).

Development, growth & differentiation·2023

Related Experiment Video

Updated: Jul 11, 2026

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
06:31

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Published on: September 27, 2018

Cyclin E2 is required for embryogenesis in Xenopus laevis.

Tetsuya Gotoh1, Noriko Shigemoto, Takeo Kishimoto

  • 1Laboratory of Cell and Developmental Biology, Graduate School of Bioscience, Tokyo Institute of Technology, Nagatsuta 4259, Yokohama, Japan.

Developmental Biology
|September 11, 2007
PubMed
Summary

Xenopus cyclin E2 is essential for embryonic development, unlike cyclin E1. This study reveals distinct roles for E-type cyclins in early development, highlighting cyclin E2

More Related Videos

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
14:50

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Published on: January 12, 2015

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting
09:32

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting

Published on: September 19, 2025

Related Experiment Videos

Last Updated: Jul 11, 2026

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
06:31

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Published on: September 27, 2018

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
14:50

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Published on: January 12, 2015

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting
09:32

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting

Published on: September 19, 2025

Area of Science:

  • Developmental Biology
  • Cell Cycle Regulation

Background:

  • E-type cyclins (E1 and E2) are typically linked to S phase entry in mammalian cells.
  • However, cyclin E's role in mammalian embryogenesis and Drosophila neural development is not fully understood, particularly during early development.

Purpose of the Study:

  • To investigate the specific roles of E-type cyclins during Xenopus embryogenesis.
  • To elucidate the distinct functions of cyclin E1 and cyclin E2 in early vertebrate development.

Main Methods:

  • Investigated Xenopus E-type cyclins (cyclin E1 and cyclin E2).
  • Inhibited cyclin E1 translation and cyclin E2 expression in developing Xenopus embryos.
  • Observed developmental outcomes following inhibition.

Main Results:

  • Inhibition of maternal cyclin E1 translation had minimal impact on Xenopus embryogenesis.
  • A zygotically expressed Xenopus cyclin E2 homologue was identified.
  • Complete inhibition of cyclin E2 expression resulted in embryonic lethality at late gastrula.
  • Partial inhibition of cyclin E2 allowed embryo survival, indicating dose-dependent effects.

Conclusions:

  • Xenopus cyclins E1 and E2 play distinct roles during embryogenesis.
  • Cyclin E2 is absolutely required for successful Xenopus embryogenesis, particularly for development up to the gastrula stage.