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Chorionic villus sampling as a source of trophoblasts.
1Perinatal Research Group, Kolling Institute of Medical Research, The University of Sydney at Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia.
Placenta
|September 11, 2007
Summary
Culturing trophoblasts from chorionic villus samples (CVS) allows for studying pregnancy disorders like pre-eclampsia. Gelatin culture proved superior for cell passaging and future research on pregnancy complications.
Area of Science:
- Reproductive Biology
- Cell Biology
- Obstetrics
Background:
- Investigating pregnancy disorders requires reliable trophoblast cell models.
- Trophoblasts from chorionic villus samples (CVS) offer a unique opportunity as pregnancy outcomes are known.
- Current methods may have limitations in providing suitable cells for extensive study.
Purpose of the Study:
- To establish and characterize trophoblast cell cultures from CVS for studying pregnancy disorders.
- To compare the efficacy of Matrigel versus gelatin as culture substrates.
- To assess the suitability of these cultured cells for future research and cryopreservation.
Main Methods:
- Chorionic villus samples (CVS) from 11-14 weeks gestation were used for explant culture.
- Outgrowing trophoblasts were cultured on Matrigel and gelatin substrates.
- Cells were characterized by morphology, cytokeratin-7, and HLA-G staining.
Main Results:
- Outgrowing trophoblasts co-expressed cytokeratin-7 and HLA-G.
- Matrigel cultures showed faster growth but cells were embedded and difficult to passage.
- Gelatin cultures allowed for trypsinization, subculturing, and successful cryopreservation.
Conclusions:
- Trophoblast cultures from CVS are a valuable resource for studying pregnancy disorders.
- Gelatin provides a superior substrate for culturing and subculturing trophoblasts compared to Matrigel.
- These established cell lines can be used for investigating conditions like pre-eclampsia.
