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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

Floating culture promotes the maintenance of hematopoietic stem cells

Teruyuki Kajiume1, Louis Yuge, Yumi Kawahara

  • 1Department of Pediatrics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. kajiume@hiroshima-u.ac.jp

FEBS Letters
|September 11, 2007
PubMed

In this study we examined the effect of the specific gravity of culture medium on the frequency of hematopoietic stem cell (HSC) maintenance. We used a newly developed high-specific-gravity media. Bone marrow cells were isolated and cultured, and HSC activity was evaluated. The number of hematopoietic progenitor/stem cells was markedly higher in the medium with high specific gravity. In high-specific-gravity media, cells did not precipitate, maintenance of HSCs was increased, and there was a concomitant accumulation of beta-catenin. This novel technique for maintaining HSC populations provides an important new tool for studies in regenerative medicine.

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