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Updated: Jul 11, 2026

Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses
Published on: November 3, 2014
The Loxtox protein family in Loxosceles intermedia (Mello-Leitão) venom
E Kalapothakis1, M Chatzaki, H Gonçalves-Dornelas
1Departamento de Biologia Geral Instituto de Ciências Biológicas; Universidade Federal de Minas Gerais, CEP 31270901 Belo Horizonte, MG, Brazil. ekalapo@icb.ufmg.br
Abstract:
We isolated cDNA sequences coding for dermonecrotic/sphingomyelinases factor proteins from the brown spider Loxosceles intermedia, here named Loxtox proteins. The amino acid sequences based on cloned cDNA of several Loxtox proteins revealed at least six distinct groups of proteins expressed in the venom gland. The level of similarity among the toxins varied from 99% to 55%. The finding of several isoforms of Loxtox in the venom of this spider may reflect an evolutionary adaptation for different prey types and reinforces the idea of an efficient mutational mechanism in the venom gland of spiders.
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