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Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Updated: May 3, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

Delineating the specificity of an IgE-encoding transcriptome.

Helena Persson¹, Mardjaneh Karbalaei Sadegh, Lennart Greiff

¹Department of Immunotechnology, Lund University, Lund, Sweden.

The Journal of Allergy and Clinical Immunology

|September 11, 2007

Summary

This summary is machine-generated.

This study investigated individual antibody specificities in grass pollen allergy. Researchers identified a restricted IgE repertoire, revealing potential targets for new allergy vaccines and immunotherapies.

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Area of Science:

Immunology
Allergy Research
Molecular Biology

Background:

Limited understanding of individual antibody specificities in grass pollen allergy.
Polyclonal antibody reactivity to timothy grass allergens is known, but individual IgE details are scarce.

Purpose of the Study:

To investigate the specific IgE antibody repertoire in a patient with grass pollen allergy.
To characterize individual antibody specificities against timothy grass allergens.

Main Methods:

Constructed a human IgE library from B cells of a timothy-allergic individual.
Screened the IgE library using phage display against six timothy grass allergens (Phl p 1, 2, 4, 5, 6, 11).

Main Results:

Identified diverse antibody fragments with specific gene usage.
Found that IgE binders were specific to their target allergens, with cross-reactivity noted between Phl p 6 and Phl p 5 due to sequence homology.
Determined the specificity of over 25% of IgE-producing transcripts in the allergic individual.

Conclusions:

The human IgE repertoire is restricted, originating from limited, related B-cell clones that recognize a finite set of antigens.
Allergen-specific antibodies can inform the development of allergy vaccines and be utilized in passive immunotherapy.