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Updated: Jul 11, 2026
Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
Published on: October 20, 2010
Scientific Impact Recognition Award. Sentinel node staging for breast cancer: intraoperative molecular pathology
Peter Blumencranz1, Pat W Whitworth, Kenneth Deck
1Breast Health Services, Morton Plant Mease Health Care, 303 Pinellas St, Ste. 310, Clearwater, FL 33756, USA.
Background:
When sentinel node dissection reveals breast cancer metastasis, completion axillary lymph node dissection is ideally performed during the same operation. Intraoperative histologic techniques have low and variable sensitivity. A new intraoperative molecular assay (GeneSearch BLN Assay; Veridex, LLC, Warren, NJ) was evaluated to determine its efficiency in identifying significant sentinel lymph node metastases (>.2 mm).
Methods:
Positive or negative BLN Assay results generated from fresh 2-mm node slabs were compared with results from conventional histologic evaluation of adjacent fixed tissue slabs.
Results:
In a prospective study of 416 patients at 11 clinical sites, the assay detected 98% of metastases >2 mm and 88% of metastasis greater >.2 mm, results superior to frozen section. Micrometastases were less frequently detected (57%) and assay positive results in nodes found negative by histology were rare (4%).
Conclusions:
The BLN Assay is properly calibrated for use as a stand alone intraoperative molecular test.
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