Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 11, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

Primary breast tumor characteristics predicting aggressive nodal involvement.

Aye Moe Thu Ma1, Monet Bowling, Marilyn Leitch

  • 1Department of Surgery, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9155, USA.

American Journal of Surgery
|September 11, 2007
PubMed
Summary

Advanced breast cancer with extensive lymph node involvement (pN3 [10] disease) shows distinct clinical features. Prognosis for these patients may be improving with current treatments.

Related Concept Videos

Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of telehealth genetic counseling on contralateral prophylactic mastectomy rates among multi-ethnic breast cancer patients.

npj women's health·2026
Same author

Breast Conserving Therapy Is a Safe Option for Women Under 40 Diagnosed With Breast Cancer.

The Journal of surgical research·2026
Same author

Ablative Preoperative Single-Fraction Radiation Dose Escalation Among Patients With Breast Cancer: A Phase 1 Nonrandomized Clinical Trial.

JAMA network open·2025
Same author

ASO Visual Abstract: Impact of Time to Surgery Post Neoadjuvant Chemotherapy on Breast Cancer Outcomes: A Retrospective Study of Patients Enrolled in the I-SPY 2 Clinical Trial.

Annals of surgical oncology·2025
Same author

ASO Visual Abstract: Impact of Neoadjuvant Chemotherapy on Surgical Outcomes and Conversion to Node-Negativity in Invasive Lobular Breast Cancer: Analysis of Molecularly High-Risk Tumors by Histologic Subtype on the I-SPY2 Clinical Trial.

Annals of surgical oncology·2025
Same author

Impact of Time to Surgery Post Neoadjuvant Chemotherapy on Breast Cancer Outcomes: A Retrospective Study of Patients Enrolled in the I-SPY 2 Clinical Trial.

Annals of surgical oncology·2025

Area of Science:

  • Oncology
  • Pathology
  • Cancer Biology

Background:

  • Breast cancer with pN3 (10) nodal involvement historically has a poor prognosis.
  • The underlying biology of aggressive lymph node metastasis (lymphotropism) in breast cancer remains poorly understood.

Purpose of the Study:

  • To compare clinical and pathological characteristics of breast cancer patients with varying stages of lymph node involvement.
  • To investigate factors associated with pN3 (10) disease and its impact on patient outcomes.

Main Methods:

  • Retrospective analysis of 1347 patients with pN0, 560 with pN1, and 100 with pN3 (10) breast cancer.
  • Comparison of clinical and pathological features across patient groups.

Main Results:

Related Experiment Videos

Last Updated: Jul 11, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

  • pN3 (10) disease correlated with larger primary tumors (T3-4), lobular histology, and inflammatory breast cancer compared to pN1 disease.
  • No association was found between pN3 (10) disease and early-onset or estrogen receptor-negative breast cancer.
  • Five- and 10-year overall survival rates for pN3 (10) patients were 64% and 21%, respectively.

    • Conclusions:

    • The prognosis for breast cancer patients with pN3 (10) disease may be improving with contemporary treatments.
    • Molecular mechanisms driving aggressive lymphotropism appear distinct from those in early-onset or ER-negative breast cancer.