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Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Published on: December 22, 2014
An improved method for single step purification of metagenomic DNA
Pushpender K Sharma1, Neena Capalash, Jagdeep Kaur
1Department of Biotechnology, Panjab University, Chandigarh, 160014, India.
Abstract:
An improved method for purification of intact metagenomic DNA from soil has been developed using Q-Sepharose, which purified the DNA from phenolic and humic acid contaminants in a single step. The entire procedure for purification took only 45 min. A total of 81% of DNA was recovered after purification and there was 84% reduction in humic acid contents. The purified DNA was readily digested with restriction enzymes and can be further used for molecular applications.
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