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Updated: Jul 11, 2026
Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology
Published on: May 23, 2025
Usefulness of a serological panel test in the assessment of gastritis in symptomatic children
Gian Luigi de Angelis1, Lucas Giovanni Cavallaro, Valentina Maffini
1Department of Paediatrics, University of Parma, Parma, Italy.
Insights
Non-invasive serological markers, including serum pepsinogen II (sPGII) and anti-Helicobacter pylori antibodies (IgG-Hp), effectively detect H. pylori infection and gastritis in children. A combined analysis of sPGII and IgG-Hp offers a reliable non-invasive assessment.
Area of Science:
- Pediatric Gastroenterology
- Infectious Diseases
- Biomarker Research
Background:
- Non-invasive detection of H. pylori-related chronic gastritis in children is crucial.
- Serum pepsinogens (sPG I, sPG II), gastrin-17 (G-17), and anti-H. pylori antibodies (IgG-Hp) are proposed as a 'serological gastric biopsy'.
Purpose of the Study:
- To assess H. pylori infection in pediatric patients using serological parameters.
- To evaluate the status of gastric mucosa in children via sPGI, sPGII, G-17, and IgG-Hp levels.
Main Methods:
- Analysis of 45 children with upper gastrointestinal symptoms.
- Upper gastrointestinal endoscopy with biopsies performed on all participants.
- Serum analysis for IgG-Hp, sPGII, sPGII, and G-17 levels.
Main Results:
- 18 children diagnosed with H. pylori-related chronic gastritis.
- Significantly higher sPGII and IgG-Hp levels in H. pylori-positive children.
- The combined product IgG-Hp x sPGII achieved 100% sensitivity and 92% specificity for H. pylori detection.
Conclusions:
- Combined analysis of sPGII and IgG-Hp is recommended for assessing H. pylori-related gastric alterations in children.
- This non-invasive panel aids in evaluating histological changes of the gastric mucosa in pediatric patients.
Background:
Non-invasive methods are advisable for the detection of Helicobacter pylori-related chronic gastritis in pediatric patients. Serum pepsinogens I and II (sPGII and sPGII), gastrin-17 (G-17) and anti-H. pylori antibodies (IgG-Hp) have been proposed as a 'serological gastric biopsy'.
Aim:
To assess H. pylori infection and to evaluate gastric mucosa status in a pediatric population by means of serological parameters such as sPGI, sPGII, G-17 and IgG-Hp.
Methods:
45 consecutively children evaluated for upper gastrointestinal symptoms were analyzed. All children were submitted to upper gastrointestinal endoscopy with biopsies. Serum samples were analyzed for IgG-Hp, sPGII, sPGI and G-17 (Biohit, Helsinki, Finland).
Results:
18 children had H. pylori-related mild or moderate non-atrophic chronic gastritis. They presented significantly higher mean levels of sPGII and of IgG-Hp than negative ones, either under or up to 10 years. sPGI showed significantly increased levels in H. pylori-positive patients only over 10 years. G-17 levels were not different between H. pylori-positive and -negative ones. The best cut-offs of IgG-Hp, sPGII and of product IgG-Hp x sPGII, to identify H. pylori infection, were 30 IU/l, 9 microg/l, and 241 IU/l x microg/l, respectively. The product IgG-Hp x sPGII identified H. pylori infection with a 100% sensitivity, 92% specificity, 90% positive predictive value and 100% negative predictive value. IgG-Hp and IgG-Hp showed a correlation (r = 0.94; p < 0.001).
Conclusions:
Combined analysis of sPGII and IgG-Hp antibody levels could be recommended as a non-invasive panel for the assessment of H. pylori-related histological alterations of gastric mucosa in childhood.
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