Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nisin biosynthesis in vitro.

Fang Cheng1, Timo M Takala, Per E J Saris

  • 1Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki, Finland.

Journal of Molecular Microbiology and Biotechnology
|September 11, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of DNA-dependent protein kinase catalytic subunit boosts rAAV transduction of polarized human airway epithelium.

Molecular therapy. Methods & clinical development·2023
Same author

Identification of Host Restriction Factors Critical for Recombinant AAV Transduction of Polarized Human Airway Epithelium.

bioRxiv : the preprint server for biology·2023
Same author

Versatile synthesis of nano-icosapods <i>via</i> cation exchange for effective photocatalytic conversion of biomass-relevant alcohols.

Chemical science·2023
Same author

Visible-Light-Driven Selective Hydrogenation of Nitrostyrene over Layered Ternary Sulfide Nanostructures.

Small (Weinheim an der Bergstrasse, Germany)·2023
Same author

Effectiveness of telemedicine-based psychosocial intervention for breast cancer patients: a systematic review and meta-analysis.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2023
Same author

Comments on "Mesenchymal Stem Cells versus Placebo for Perianal Fistulizing Crohn's Disease: A Systemic Review and Meta-Analysis".

Surgical innovation·2023
Same journal

Immunogenicity Evaluation of Chimeric Subunit Vaccine Comprising Adhesion Coli Surface Antigens from Enterotoxigenic Escherichia coli.

Journal of molecular microbiology and biotechnology·2020
Same journal

Study of the Enzymatic Capacity of Kluyveromyces marxianus for the Synthesis of Esters.

Journal of molecular microbiology and biotechnology·2020
Same journal

Vaccination of Mice with Listeria ivanovii Expressing the Truncated M Protein of Porcine Reproductive and Respiratory Syndrome Virus Induces both Antigen-Specific CD4+ and CD8+ T Cell-Mediated Immunity.

Journal of molecular microbiology and biotechnology·2020
Same journal

Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus.

Journal of molecular microbiology and biotechnology·2020
Same journal

Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp. H11.

Journal of molecular microbiology and biotechnology·2020
Same journal

Characterization of Alanine Dehydrogenase and Its Effect on Streptomyces coelicolorA3(2) Development in Liquid Culture.

Journal of molecular microbiology and biotechnology·2019
See all related articles

Researchers developed an in vitro system to produce the lantibiotic nisin using the Rapid Translation System (RTS). This method successfully modified the nisin precursor, yielding active nisin, and offers potential for creating novel nisin variants and stabilizing therapeutic peptides.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Nisin, a lantibiotic, is synthesized by Lactococcus lactis.
  • Biosynthesis involves NisB (dehydration) and NisC (lanthionine formation).
  • In vivo modification typically occurs membrane-associated with NisT transporter.

Purpose of the Study:

  • To express and characterize nisin precursor modification enzymes (NisB, NisC) in vitro.
  • To investigate the necessity of membrane association and NisT for nisin modification.
  • To establish a cell-free system for nisin production and engineering.

Main Methods:

  • In vitro expression of nisA, nisB, and nisC genes using the Rapid Translation System (RTS).
  • Analysis of the RTS mixture for nisin precursor modification.

Related Experiment Videos

  • Enzymatic treatment with trypsin to obtain active nisin.
  • Assessment of zinc dependency for NisC activity.

    • Main Results:

    • Fully modified nisin precursor was successfully synthesized in vitro.
    • Active nisin was obtained after trypsin treatment.
    • Zinc supplementation was essential for NisC enzyme function.
    • Nisin precursor modification occurred independently of membrane association and the NisT transporter.

    Conclusions:

    • The cell-free system enables nisin precursor modification without membrane association or NisT.
    • This system facilitates the production of novel nisin variants not achievable in vivo.
    • The NisB and NisC enzymes can be utilized to incorporate thioether rings into peptides, enhancing stability for therapeutic applications.