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Updated: Jul 11, 2026

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
Published on: September 28, 2018
CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1
Ikuko Hayashi1, Michael J Plevin, Mitsuhiko Ikura
1Division of Signaling Biology, Ontario Cancer Institute, University of Toronto, 101 College Street, Toronto, Ontario, M5G 1L7, Canada. ihayashi@uhnres.utoronto.ca
Abstract:
CLIP170 and p150(Glued) localize to the plus ends of growing microtubules. Using crystallography and NMR, we show that autoinhibitory interactions within CLIP170 use the same binding determinants as CLIP170's intermolecular interactions with p150(Glued). These interactions have both similar and distinct features when compared with the p150(Glued)-EB1 complex. Our data thus demonstrate that regulation of microtubule dynamics by plus end-tracking proteins (+TIPs) occurs through direct competition between homologous binding interfaces.
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