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Improved immunoblotting for the detection and quantitative analysis of antibodies to human immunodeficiency virus
P Portincasa1, G Conti, M Gerloni
1Institute of Microbiology, University of Parma, Medical School, Italy.
European Journal of Epidemiology
|November 1, 1991
Abstract:
Detection of antibodies to single HIV-1 proteins was performed by modified Western-blotting procedure. The use of [35S]-Streptavidin instead of Avidin-peroxidase conjugate greatly enhances the sensitivity of the method and enables useful quantitative analysis of results.