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Physiological studies on the utilization of oleic acid by Saccharomyces cerevisiae in relation to microbody
M E Evers1, J Höhfeld, W H Kunau
1Laboratory for Electron Microscopy, University of Groningen, Haren, The Netherlands.
Abstract:
We studied the influence of specific growth conditions on the induction of beta-oxidation enzymes and rate of microbody proliferation in S. cerevisiae by oleic acid. Of all conditions tested, highest enzyme levels and microbody numbers were achieved in glucose-limited continuous cultures, supplemented with oleic acids second carbon source. Comparable enzyme levels were observed in identical cultures of peroxisome-deficient (pas) mutants of S. cerevisiae. These experiments showed chemostat cultures on glucose/oleic acid mixtures to be a method of choice for future studies on microbody biogenesis/assembly in constructed, conditional pas mutants.