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Related Experiment Videos

Mouse proacrosin gene: nucleotide sequence, diploid expression, and chromosomal localization.

H Kremling1, S Keime, K Wilhelm

  • 1Institut für Humangenetik der Universität, Göttingen, Federal Republic of Germany.

Genomics
|December 1, 1991
PubMed
Summary

Researchers isolated the mouse proacrosin gene, revealing its six-exon structure and diploid expression during spermatogenesis. This contrasts with haploid expression in other species, offering new insights into sperm development.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Reproductive Biology

Background:

  • Acrosin, a sperm serine proteinase, is synthesized as a zymogen (proacrosin) from a preproenzyme (preproacrosin).
  • Preproacrosin contains a hydrophobic leader sequence and its cDNA sequences are known for human, boar, and mouse.

Purpose of the Study:

  • To isolate and characterize the gene encoding mouse proacrosin.
  • To elucidate the gene structure, regulatory elements, and chromosomal location of mouse proacrosin.
  • To investigate the expression pattern of the proacrosin gene during spermatogenesis in mice.

Main Methods:

  • Isolation of the mouse proacrosin gene from a mouse cosmid library using cDNA probes.
  • Analysis of gene structure, including exon-intron organization and transcription initiation site.

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  • Primer extension analysis to determine the transcription initiation site and identify TATA and CAAT boxes.
  • Mapping the gene location on mouse chromosome 15 and its syntenic group on human chromosome 22.

    • Main Results:

    • The mouse proacrosin gene comprises six exons with an intron in the 5'-untranslated region.
    • The transcription initiation site was identified 581 nucleotides upstream of the ATG codon, with TATA and CAAT boxes located at -26 and -97, respectively.
    • Active-site residues (His, Asp, Ser) are encoded by separate exons (E2, E3, E5), with the proline-rich domain in exon 5.
    • The proacrosin gene is located on mouse chromosome 15 (bands E/F) and is syntenic with human chromosome 22 (q13-qter).
    • Mouse proacrosin gene expression during spermatogenesis is diploid, unlike the haploid expression in bull, boar, and rat.

    Conclusions:

    • The study successfully isolated and characterized the mouse proacrosin gene, detailing its structure and regulatory regions.
    • The findings reveal a unique diploid expression pattern of the proacrosin gene in mice during spermatogenesis.
    • This research provides a foundation for understanding the genetic regulation of sperm function and potential implications for male fertility.