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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Electron imaging of pyrrhotite superstructures.

L Pierce, P R Buseck

    Science (New York, N.Y.)
    |December 27, 1974
    PubMed
    Summary

    High-resolution electron microscopy reveals structural heterogeneity in natural pyrrhotites. Superstructures arise from ordered or disordered sequences of antiphase domains, explaining different pyrrhotite types.

    Area of Science:

    • Mineralogy and crystallography
    • Materials science

    Background:

    • Pyrrhotite, an iron sulfide mineral, exhibits complex structural variations.
    • Understanding these variations is crucial for interpreting its properties and formation.

    Purpose of the Study:

    • To investigate the crystallographic information of natural pyrrhotites at the unit cell scale.
    • To elucidate the structural basis of pyrrhotite superstructures using high-resolution electron microscopy.

    Main Methods:

    • High-resolution electron microscopy (HREM) for atomic-scale imaging.
    • Analysis of crystallographic data derived from HREM.

    Main Results:

    • Structural heterogeneity is a prominent feature in natural pyrrhotite samples.

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  • Reported superstructures are consistently explained by an antiphase domain model.
  • The 5C pyrrhotite superstructure is characterized by an ordered sequence of antiphase domains.
  • The higher-temperature NC type pyrrhotite exhibits a disordered sequence of antiphase domains.
  • Conclusions:

    • The antiphase domain model provides a unified framework for understanding pyrrhotite superstructures.
    • The ordering or disordering of these domains dictates the specific superstructure type and its temperature dependence.