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Naphthol and lens.

M S Murthy1

  • 1Dept. of Biochemistry, Department of Atomic Energy Hospital, Kalpakkam.

Indian Journal of Medical Sciences
|September 1, 1991
PubMed
Summary
This summary is machine-generated.

Naphthalene metabolites, alpha and beta naphthols, reduced sheep lens protease activity and increased amino acid leakage. High galactose levels partially counteracted these effects, suggesting a role in cataract development.

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Area of Science:

  • Biochemistry
  • Ophthalmology
  • Toxicology

Background:

  • Naphthalene is a known cataractogenic agent.
  • Alpha and beta naphthols are primary metabolites of naphthalene.
  • Lens protein proteases and inhibitors play roles in maintaining lens clarity.

Purpose of the Study:

  • To investigate the effects of alpha and beta naphthols on sheep lens proteases and their inhibitors.
  • To explore the impact of these metabolites on amino acid efflux from the lens.
  • To determine if galactose influences these effects.

Main Methods:

  • Incubation of sheep lenses with alpha and beta naphthols.
  • Assay of protease and inhibitor activities in lens proteins.
  • Measurement of free amino acid efflux from the lenses.

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  • Evaluation of the effects of high galactose concentrations.

    • Main Results:

    • Alpha and beta naphthols significantly reduced protease activities in sheep lenses.
    • No significant effect was observed on the inhibitor activities of lens proteins.
    • Increased efflux of free amino acids from the lenses was noted.
    • High concentrations of tissue galactose partially retarded the amino acid efflux.

    Conclusions:

    • Alpha and beta naphthols directly impact lens proteases, potentially contributing to cataract formation.
    • The observed amino acid efflux and its modulation by galactose warrant further investigation in naphthalene-induced cataracts.