Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterization of the thylakoid membranes of the tobacco aurea mutant Su/su and of three green revertant plants.

Planta·2013

Same author

In vitro selection of Nicotiana sylvestris variants with limited resistance to TMV.

Plant cell reports·2013

Same author

ABA prevents the second cytokinin-mediated event during the induction of shoot buds in the moss Funaria hygrometrica.

American journal of botany·2000

Same author

Osteogenesis from cultured chick periostea has a specific requirement for chloride.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2000

Same author

Phenylurea cytokinins assayed for induction of shoot buds in the moss Funaria hygrometrica.

American journal of botany·1999

Same author

Exceptional segregation of a selectable marker (KanR) in Arabidopsis identifies genes important for gametophytic growth and development.

Genetics·1998

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

A morphogenetically competent soybean suspension culture.

M L Christianson, D A Warnick, P S Carlson

Science (New York, N.Y.)

|November 11, 1983

Summary

Researchers developed a stable Glycine max (soybean) suspension culture capable of producing plantlets. This method utilizes specific selection and media modifications for efficient embryoid development and plant regeneration.

Area of Science:

Plant Biotechnology
Somatic Embryogenesis
Crop Improvement

Background:

Developing efficient plant tissue culture systems is crucial for crop improvement.
Glycine max (soybean) regeneration can be challenging.
Somatic embryogenesis offers a pathway for mass propagation.

Purpose of the Study:

To establish a morphogenetically competent suspension culture of Glycine max cv. Mitchell.
To enable plantlet development from embryoids derived from the suspension culture.
To ensure the long-term viability and maintenance of the suspension culture.

Main Methods:

Derivation of suspension culture from embryonic axes of Glycine max cv. Mitchell.
Visual selection for nonfriable, embryo-like structures.

More Related Videos

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

Published on: June 30, 2023

Polysome Purification from Soybean Symbiotic Nodules

Polysome Purification from Soybean Symbiotic Nodules

Published on: July 1, 2022

Related Experiment Videos

Last Updated: Jul 11, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

Published on: June 30, 2023

Polysome Purification from Soybean Symbiotic Nodules

Polysome Purification from Soybean Symbiotic Nodules

Published on: July 1, 2022

Recurrent selection involving 2,4-dichlorophenoxyacetic acid (2,4-D) exposure and withdrawal.

Modification of Murashige and Skoog medium with 20 mM ammonium citrate.

Main Results:

A stable, morphogenetically competent suspension culture was successfully established.
Embryoids derived from the suspension culture demonstrated the capacity for complete plantlet development.
The suspension culture proved maintainable through serial subculture.

Conclusions:

The developed suspension culture protocol provides a robust method for Glycine max regeneration.
This system holds potential for genetic manipulation and large-scale propagation of soybean.
Optimized culture conditions facilitate efficient somatic embryogenesis and plantlet recovery.