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Updated: Jul 11, 2026

Fabrication of Micro-Patterned Chip with Controlled Thickness for High-Throughput Cryogenic Electron Microscopy
Published on: April 21, 2022
Fabrication of fritless chromatographic microchips packed with conventional reversed-phase silica particles
Attila Gaspar1, Menake E Piyasena, Frank A Gomez
1Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032-8202, USA.
Abstract:
This paper describes the development and study of a disposable and inexpensive microfluidic chip, fabricated from poly(dimethylsiloxane) (PDMS) incorporating conventional chromatographic reversed-phase silica particles (C18) without the use of frits, permanent physical barriers, tapers, or restrictors. The packing of C18 modified silica particles into the microfluidic channels is made possible by the hydrophobic nature and excellent elasticity of PDMS. Keystone-, clamping-, and anchor-effects provide the stability and the compactness of the packing and attenuated wall-effects were observed.
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