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Inhibitory effects of Maharishi-4 and Maharishi-5 on microsomal lipid peroxidation
C Dwivedi1, H M Sharma, S Dobrowski
1College of Pharmacy, South Dakota State University, Brookings 57007.
Abstract:
The effects of Maharishi-4 (M-4) and Maharishi-5 (M-5) on microsomal lipid peroxidation were examined in vitro. Rat liver microsomes were incubated with an NADPH-generating system or with sodium ascorbate and an ADP-iron complex to stimulate enzymatic or nonenzymatic lipid peroxidation respectively. Alcoholic or aqueous extracts of M-4 or M-5, when added to these incubation systems, inhibited hepatic microsomal lipid peroxidation in a concentration-dependent manner. The aqueous extract of M-4 was the most effective antiperoxidant in these systems. A 10% (w/v) aqueous extract of M-4 inhibited ascorbate or NADPH-induced lipid peroxidation by approximately 50% when added at volumes of 8 microliters and 3.5 microliters respectively to the incubation mixtures (total incubation volume, 2 ml). These findings suggest that M-4 and M-5, by virtue of their antiperoxidant properties, may be useful in the treatment of free radical-linked drug toxicities and disease states.
Insights
Maharishi-4 (M-4) and Maharishi-5 (M-5) extracts showed significant antiperoxidant activity in rat liver microsomes. The aqueous extract of M-4 was most effective, inhibiting lipid peroxidation and suggesting potential therapeutic applications.
Area of Science:
- Biochemistry
- Pharmacology
- Oxidative Stress Research
Background:
- Lipid peroxidation is a key process in cellular damage.
- Free radical-linked diseases and drug toxicities are significant health concerns.
- Natural compounds are being investigated for their antioxidant properties.
Purpose of the Study:
- To evaluate the in vitro antiperoxidant effects of Maharishi-4 (M-4) and Maharishi-5 (M-5) extracts.
- To determine the efficacy of different M-4 and M-5 extracts (alcoholic vs. aqueous) on lipid peroxidation.
- To assess the potential of M-4 and M-5 in mitigating oxidative damage.
Main Methods:
- Rat liver microsomes were used as the experimental model.
- Enzymatic (NADPH-generating system) and nonenzymatic (sodium ascorbate/ADP-iron) lipid peroxidation were induced.
- Concentration-dependent effects of M-4 and M-5 extracts on lipid peroxidation were measured.
Main Results:
- Both M-4 and M-5 extracts inhibited hepatic microsomal lipid peroxidation in a concentration-dependent manner.
- The aqueous extract of M-4 demonstrated the most potent antiperoxidant activity.
- A 10% aqueous M-4 extract significantly reduced both ascorbate- and NADPH-induced lipid peroxidation.
Conclusions:
- M-4 and M-5 possess significant in vitro antiperoxidant properties.
- The aqueous extract of M-4 is a particularly effective inhibitor of lipid peroxidation.
- These findings suggest M-4 and M-5 may be beneficial in treating conditions associated with free radical damage.