Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Hypoxia01:23

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deciphering the mechanisms of oxygen transfer into a wine bottle.

Science advances·2026
Same author

Magnetic resonance imaging-guided radiotherapy for prostate cancer: A systematic review of the literature.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2026
Same author

Bevacizumab for Radiation-Induced (Radiculo)plexopathy.

European journal of neurology·2026
Same author

Glycine solvation in supercooled water.

The Journal of chemical physics·2026
Same author

Short-term Androgen Deprivation Therapy and High-dose Radiotherapy in Intermediate- and High-risk Localized Prostate Cancer: Results from the GETUG 14 Randomized Phase 3 Trial.

European urology·2025
Same author

Evaluation of 30-day mortality after palliative radiotherapy of 8Gy in a single fraction as a quality criteria of patient management in a university hospital.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2025

Related Experiment Video

Updated: Jul 11, 2026

Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

[Hypoxia and angiogenesis].

Jean-Marc Simon1

  • 1Service d'oncologie radiothérapique, Groupe hospitalier Pitié-Salpêtrière, APHP, 47-83, boulevard de l'Hôpital, 75651 Paris Cedex 13. jean-marc.simon@psl.aphp.fr

Bulletin Du Cancer
|September 13, 2007
PubMed
Summary

Hypoxic tumors have a worse prognosis because oxygen-deprived cells activate hypoxia-inducible factor 1-alpha (HIF1alpha). This protein promotes angiogenesis, increasing blood supply to tumors and aiding their growth and therapy resistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Physiology

Context:

  • Solid tumors often contain hypoxic regions, creating an environment linked to increased malignancy and poorer patient outcomes.
  • Oxygen-deprived tumor cells activate specific molecular pathways to survive, adapt, and resist therapeutic interventions.
  • Hypoxia triggers cellular stress responses, with Hypoxia-Inducible Factor 1-alpha (HIF1alpha) being a central regulator.

Purpose:

  • To elucidate the role of HIF1alpha in mediating the cellular response to tumor hypoxia.
  • To understand how hypoxia-driven gene expression, particularly angiogenesis, influences tumor progression.
  • To explore the dual role of angiogenesis in both physiological adaptation and pathological tumor growth.

Summary:

  • Hypoxia within solid tumors activates HIF1alpha, a transcription factor that upregulates genes essential for adaptation.

More Related Videos

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Induction of Hypoxia in Living Frog and Zebrafish Embryos
08:01

Induction of Hypoxia in Living Frog and Zebrafish Embryos

Published on: June 26, 2017

Related Experiment Videos

Last Updated: Jul 11, 2026

Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Induction of Hypoxia in Living Frog and Zebrafish Embryos
08:01

Induction of Hypoxia in Living Frog and Zebrafish Embryos

Published on: June 26, 2017

  • A key HIF1alpha target gene, vascular endothelial growth factor (VEGF), stimulates angiogenesis to enhance blood flow to hypoxic regions.
  • While crucial for development and tissue repair, hypoxia-induced angiogenesis also promotes tumor growth and therapeutic resistance.

    • Impact:

    • Understanding the HIF1alpha-VEGF axis in hypoxia provides insights into tumor biology and potential therapeutic targets.
    • This pathway's role in promoting angiogenesis highlights its significance in malignant progression and metastasis.
    • Targeting hypoxia-induced angiogenesis could offer novel strategies to overcome treatment resistance in solid tumors.