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Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Corticosterone impairs dendritic cell maturation and function.

Michael D Elftman1, Christopher C Norbury, Robert H Bonneau

  • 1Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey, PA 17033-0850, USA.

Immunology
|September 13, 2007
PubMed
Summary

Stress hormone corticosterone (CORT) impairs dendritic cell (DC) maturation, hindering their ability to initiate adaptive immunity. This compromises the immune system

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Area of Science:

  • Immunology
  • Neuroendocrinology

Background:

  • Dendritic cells (DCs) are crucial for initiating adaptive immune responses.
  • DC maturation is essential for effective T-cell priming, including cytotoxic T lymphocytes (CTLs).
  • Neuroendocrine mediators, like corticosterone (CORT), influence immune function, but their impact on DCs is less understood.

Purpose of the Study:

  • To investigate the effects of corticosterone (CORT) on dendritic cell (DC) maturation and function.
  • To determine if CORT compromises the ability of DCs to prime T-cell responses.

Main Methods:

  • Exposure of DCs to physiologically relevant concentrations of CORT.
  • Assessment of DC maturation markers and cytokine production (IL-6, IL-12, TNF-α) after LPS stimulation.
  • Evaluation of DC's capacity to prime naive CD8(+) T cells in vivo.

Main Results:

  • CORT, acting via glucocorticoid receptors, functionally compromised DC maturation.
  • CORT-treated DCs remained immature phenotypically and functionally after LPS stimulation.
  • CORT-treated DCs showed impaired production of IL-6, IL-12, and TNF-α.
  • CORT treatment significantly reduced the ability of DCs to prime naive CD8(+) T cells in vivo.

Conclusions:

  • Physiologically relevant CORT concentrations impair DC maturation and function.
  • Compromised DC maturation by CORT may underlie stress-associated immunosuppression.
  • These findings highlight a mechanism by which stress hormones impact adaptive immunity initiation.