Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aptameric enzyme subunit for homogeneous DNA sensing.

Kazunori Ikebukuro1, Wataru Yoshida, Koji Sode

  • 1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan. ikebu@cc.tuat.ac.jp

Biotechnology Letters
|September 13, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Silico Screening Using Freely Available Computational Tools to Generate Novel Peptide/Protein Pairs for Protein-Protein Ligation.

Chembiochem : a European journal of chemical biology·2026
Same author

Convenient and sensitive detection of viable Escherichia coli employing a sequential reaction between antibody-enzyme complexes.

Biosensors & bioelectronics·2026
Same author

Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model.

Analytical methods : advancing methods and applications·2026
Same author

Turning on Protein Function Inhibited by DNA Aptamers Employing a Covalent DNA-Binding Protein.

ACS nanoscience Au·2026
Same author

Creation of an Engineered Oxygen-Insensitive L-Glutamate Oxidase for the Application of Electrochemical L-Glutamate Sensors.

International journal of molecular sciences·2026
Same author

A bioreference electrode; a reference electrode which shifts its redox potential symmetrically with the working electrode to develop reliable bioelectrochemical sensors.

Biosensors & bioelectronics·2026

Researchers created an aptameric enzyme subunit (AES) for homogeneous DNA detection. This novel biosensor detects target DNA by measuring recovered enzyme activity upon target binding.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers are nucleic acid-based recognition elements with high specificity.
  • Enzyme-linked aptasensors offer sensitive detection methods.
  • Homogeneous detection assays simplify sample processing and reduce assay time.

Purpose of the Study:

  • To develop a novel aptameric enzyme subunit (AES) for homogeneous DNA detection.
  • To engineer an artificial enzyme subunit capable of detecting target DNA.
  • To demonstrate the feasibility of using AES for sensitive DNA quantification.

Main Methods:

  • Constructed an AES by conjugating a probe DNA to a thrombin-inhibiting aptamer.
  • Investigated the inhibitory activity of the modified aptamer.

Related Experiment Videos

  • Assessed the recovery of inhibitory activity upon hybridization with target DNA.
  • Quantified target DNA by measuring thrombin activity in a homogeneous solution.

    • Main Results:

    • The engineered AES exhibited reduced inhibitory activity compared to the original aptamer.
    • Hybridization with target DNA restored the aptamer's inhibitory activity.
    • The AES successfully detected target DNA in a homogeneous solution by monitoring thrombin activity.
    • The developed method provides a sensitive and homogeneous approach for DNA detection.

    Conclusions:

    • The aptameric enzyme subunit (AES) is a viable tool for homogeneous DNA detection.
    • AES technology offers a promising platform for developing novel biosensors.
    • This approach enables sensitive and specific detection of target nucleic acids.