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Updated: Jul 11, 2026
Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
Published on: November 16, 2012
Development of a bacterial surface display of hexahistidine peptide using CS3 pili for bioaccumulation of heavy
Behnaz Saffar1, Bagher Yakhchali, Mehdi Arbabi
1National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Abstract:
A novel cell surface display system for metal uptake was developed using CS3 pili of enterotoxigenic Escherichia coli, which is a suitable system for display of heterologous peptides. The recombinant bacteria producing the hybrid pili containing the hexahistidine peptide accumulated high concentrations of Cd(2+) and Ni(2+) at 656.2 and 276.5 nmol per mg dry weight of bacterial cell, respectively. The recombinant bacteria may be useful in water and waste water treatment.
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