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Updated: Jul 11, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU
P G Higgins1, H Wisplinghoff, O Krut
1Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.
Abstract:
A new PCR-based method that exploits differences in gyrB gene sequences was developed to distinguish between Acinetobacter baumannii and Acinetobacter genomic sp. 13TU. Among 118 clinical and reference Acinetobacter strains, 102 of which were previously speciated by amplified rDNA restriction analysis as belonging to the Acinetobacter calcoaceticus-A. baumannii complex, the method correctly identified 31 A. baumannii and 54 Acinetobacter genomic sp. 13TU isolates to the species level. The method was rapid, specific and easy to interpret.
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