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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: Jul 11, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Chemical probes shed light on protein function.

Helen M O'Hare1, Kai Johnsson, Arnaud Gautier

  • 1Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

Current Opinion in Structural Biology
|September 14, 2007
PubMed
Summary

Site-specific protein labeling uses synthetic dyes and genetic tags for advanced live cell imaging. Recent innovations enable non-invasive visualization of protein dynamics and localization in biological research.

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Last Updated: Jul 11, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
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Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Imaging

Background:

  • Live cell imaging is crucial for understanding dynamic biological processes.
  • Site-specific protein labeling allows precise visualization of protein behavior within cells.
  • Traditional methods often face limitations in specificity and invasiveness.

Purpose of the Study:

  • To review recent advancements in site-specific protein labeling techniques.
  • To highlight novel protein tags and their applications in live cell imaging.
  • To showcase the utility of these methods in addressing complex biological questions.

Main Methods:

  • Utilizing protein or peptide tags fused to target proteins.
  • Employing synthetic dyes and small molecule probes for labeling.
  • Applying advanced imaging techniques for non-invasive observation.

Main Results:

  • Significant progress in site-specific labeling methodologies over the past two years.
  • Development and publication of numerous new protein tags.
  • Creative application of established techniques to solve challenging biological problems.

Conclusions:

  • Site-specific protein labeling is a rapidly advancing field with broad applicability.
  • These techniques offer powerful tools for studying protein localization and dynamics.
  • Future research will likely see further innovative applications in live cell imaging.