Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Doxycycline promotes proteasome fitness in the central nervous system.

Scientific reports·2021
Same author

Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast.

Methods in molecular biology (Clifton, N.J.)·2021
Same author

XPG-related nucleases are hierarchically recruited for double-stranded rDNA break resection.

The Journal of biological chemistry·2019
Same author

Histone H3G34R mutation causes replication stress, homologous recombination defects and genomic instability in <i>S. pombe</i>.

eLife·2017
Same author

DNA Topoisomerase II modulates acetyl-regulation of cohesin-mediated chromosome dynamics.

Current genetics·2017
Same author

An acetyltransferase-independent function of Eso1 regulates centromere cohesion.

Molecular biology of the cell·2016

Related Experiment Video

Updated: Jul 11, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Turning off the G2 DNA damage checkpoint.

Teresa M Calonge1, Matthew J O'Connell

  • 1Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA.

DNA Repair
|September 14, 2007
PubMed
Summary

Cells activate DNA damage checkpoints to halt cell cycle progression, allowing DNA repair. This review focuses on the signals that terminate the G2 DNA damage checkpoint, enabling cell cycle resumption and mitosis.

Area of Science:

  • Cellular biology
  • Molecular biology
  • Genetics

Background:

  • DNA damage triggers cell cycle checkpoints to prevent genomic instability.
  • The G2 DNA damage checkpoint, mediated by Chk1 kinase, inhibits entry into mitosis.
  • Understanding checkpoint regulation is crucial for cell survival and proliferation.

Purpose of the Study:

  • To review current knowledge on G2 DNA damage checkpoint termination.
  • To elucidate the signals that inactivate Chk1 and its regulators.
  • To provide insights into the resumption of cell cycling after DNA damage.

Main Methods:

  • Literature review of studies on DNA damage response pathways.
  • Analysis of signaling cascades controlling Chk1 activity.
  • Comparative analysis across conserved species.

More Related Videos

Cell Cycle-specific Measurement of &#947;H2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
08:21

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Published on: September 1, 2019

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Related Experiment Videos

Last Updated: Jul 11, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Cell Cycle-specific Measurement of &#947;H2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
08:21

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Published on: September 1, 2019

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Main Results:

  • Detailed understanding of Chk1 activation mechanisms exists.
  • Checkpoint termination signals are less understood but critical for cell survival.
  • Specific termination pathways involve phosphatases and negative feedback loops.

Conclusions:

  • Efficient termination of the G2 DNA damage checkpoint is essential for cell cycle progression and mitosis.
  • Further research into termination signals will reveal new therapeutic targets.
  • Conserved mechanisms highlight the fundamental importance of this process.