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Related Experiment Video

Updated: Jul 11, 2026

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
08:18

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Published on: March 22, 2010

Isolation murine mesenchymal stem cells by positive selection.

Samad Nadri1, Masoud Soleimani

  • 1Stem Cells Department, Stem Cell Technology, Co, Tehran, Iran.

In Vitro Cellular & Developmental Biology. Animal
|September 14, 2007
PubMed
Summary

Researchers efficiently isolated homogenous mesenchymal stem cells (MSCs) from mouse bone marrow using CD34(+) positive selection. These purified MSCs exhibit plastic adherence and differentiate into bone and fat cells in vitro.

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Area of Science:

  • Stem Cell Biology
  • Hematology
  • Cellular and Molecular Medicine

Background:

  • Isolating mesenchymal stem cells (MSCs) from mouse bone marrow using traditional plastic adherence is challenging due to contamination by hematopoietic and non-MSC populations.
  • The CD34 antigen is a marker often associated with hematopoietic stem cells, but its role in MSC isolation requires further investigation.

Purpose of the Study:

  • To develop an efficient protocol for isolating homogenous populations of mesenchymal stem cells (MSCs) from C57BL/6 mouse bone marrow.
  • To characterize the isolated CD34(+) cells for their mesenchymal stem cell properties, including surface marker expression and differentiation potential.

Main Methods:

  • Positive selection of CD34(+) cells from mouse bone marrow using magnetic beads conjugated to anti-CD34 antibodies.

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Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
09:47

Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs

Published on: June 17, 2016

Related Experiment Videos

Last Updated: Jul 11, 2026

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
08:18

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Published on: March 22, 2010

Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
09:47

Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs

Published on: June 17, 2016

  • Culture of positively selected cells in plastic adherent flasks.
  • Flow cytometry analysis to assess surface marker expression (CD34, CD44, Sca-1, Vcam-1, CD11b, CD45).
  • In vitro induction of differentiation into osteoblastic and adipogenic lineages.

    • Main Results:

    • Homogenous populations of fibroblast-like CD34(+) cells were successfully isolated within the first passage (1 week).
    • The purified cells expressed MSC markers CD34, CD44, Sca-1, and Vcam-1, while lacking hematopoietic markers CD11b and CD45.
    • Isolated cells demonstrated the capacity for osteogenic and adipogenic differentiation in vitro.

    Conclusions:

    • The described protocol enables efficient isolation of homogenous mesenchymal stem cells (MSCs) from C57BL/6 mouse bone marrow.
    • Murine bone marrow-derived CD34(+) cells with plastic adherence and trilineage differentiation potential are identified as MSCs.