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Updated: Jul 11, 2026
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Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection
Published on: June 8, 2020
LRIG1 expression in colorectal cancer
Ingrid Ljuslinder1, Irina Golovleva, Richard Palmqvist
1Department of Radiation Sciences, Oncology, Umeå University Hospital, Umeå University, Umeå, Sweden. ingrid.ljuslinder@onkologi.umu.se
Acta Oncologica (Stockholm, Sweden)
|September 14, 2007
Summary
Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) expression in colorectal cancer shows significant heterogeneity. Its expression levels were not correlated with epidermal growth factor receptor (EGFR) levels or gene copy number in this study.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Colorectal cancer frequently exhibits epidermal growth factor receptor (EGFR) overexpression, linked to poor prognosis.
- Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) is an integral membrane protein that negatively regulates receptor tyrosine kinases, including EGFR.
- LRIG1 is widely expressed across various human tissues.
Purpose of the Study:
- To investigate the expression of LRIG1 in colorectal cancer tissues.
- To determine the relationship between LRIG1 expression and EGFR expression in colorectal cancer.
- To assess the correlation between LRIG1 gene dosage and its protein expression.
Main Methods:
- Western blot analysis of LRIG1 protein in tumor and adjacent non-neoplastic tissues from 30 colorectal cancer patients.
- Immunohistochemistry to assess EGFR expression.
- Fluorescence in situ hybridization (FISH) to analyze LRIG1 gene copy number in tumors and cell lines.
Main Results:
- LRIG1 protein expression was upregulated in 43% and downregulated in 43% of colorectal cancers compared to adjacent tissues.
- No significant correlation was found between LRIG1 protein levels and EGFR expression.
- Increased LRIG1 gene copy number was observed in only one of nine tumors; cell lines showed two copies, indicating expression heterogeneity is not gene-dosage related.
Conclusions:
- Colorectal cancer exhibits significant heterogeneity in LRIG1 protein expression.
- LRIG1 expression in colorectal cancer does not correlate with EGFR expression or LRIG1 gene copy number.
- Further research is needed to clarify the biological and clinical significance of altered LRIG1 expression in colorectal cancer.
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