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1A and 3D gene sequences of coxsackievirus B3 strain CC: variation and phylogenetic analysis

Ming-Yu Liu1, Dong-Lai Wu, Ni-Hong Liu

  • 1Geriatric Department, 2nd Hospital of Harbin Medical University, Harbin 150086, People's Republic of China.

DNA Sequence : the Journal of DNA Sequencing and Mapping
|September 14, 2007
PubMed

Insights

This study sequenced Coxsackievirus B3 strain CC (CVB3-CC), revealing its genetic makeup and evolutionary links. Phylogenetic analysis suggests CVB3-CC is closely related to the CVB3 Nancy strain, with evidence of viral recombination.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Coxsackievirus B3 (CVB3) is a primary cause of viral myocarditis.
  • CVB3 strain CC (CVB3-CC) was isolated in China, but its genetic data were previously unavailable.

Purpose of the Study:

  • To sequence and analyze the 1A and 3D gene regions of CVB3-CC.
  • To determine the evolutionary relationship of CVB3-CC with other CVB3 strains and human enterovirus B (HEV-B).
  • To investigate potential genetic recombination among CVB3 strains.

Main Methods:

  • Sequencing of the 1A and 3D gene regions of CVB3-CC.
  • Phylogenetic analysis using reference CVB3 strains and HEV-B prototype strains.
  • Sequence variation analysis of the 1A and 3D gene regions.

Main Results:

  • The 1A gene region of CVB3-CC has 207 nucleotides (69 amino acids); the 3D region has 1386 nucleotides (462 amino acids).
  • The 3D gene region exhibited less variation compared to the 1A region.
  • Phylogenetic analysis placed CVB3-CC close to CVB3 Nancy, suggesting a close evolutionary relationship.
  • Topological incongruity between protein gene trees indicated viral recombination.

Conclusions:

  • CVB3-CC shares a close evolutionary relationship with the CVB3 Nancy strain.
  • Evidence of recombination among CVB3 strains was observed.
  • This study provides crucial genetic data for CVB3-CC, aiding in understanding viral evolution and pathogenesis.

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