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Amyloid enhancing factor (AEF). Isolation and biochemical and pathological characteristics.
T Yokota1, M Takahashi, T Ishihara
1First Department of Pathology, Yamaguchi University School of Medicine, Japan.
Acta Pathologica Japonica
|November 1, 1991
Summary
Amyloid enhancing factor (AEF) accelerates amyloid deposition. This glycoprotein, associated with neutrophils, was purified and characterized, revealing its role in amyloidosis.
Area of Science:
- Biochemistry
- Immunology
- Hematology
Background:
- Amyloid deposition is a hallmark of several diseases.
- Neutrophils are implicated in inflammatory processes.
- The factors influencing amyloid deposition require further investigation.
Purpose of the Study:
- To identify and characterize the factor responsible for accelerating amyloid deposition.
- To determine the cellular origin and biochemical nature of this factor.
Main Methods:
- Induction of amyloid deposition in mice.
- Isolation and purification of amyloid enhancing factor (AEF) using ion-exchange chromatography and HPLC.
- Biochemical analysis of AEF, including amino acid sequencing and gas chromatography.
- Immunoelectron microscopy to localize AEF within neutrophils.
Main Results:
- Neutrophil injection and their supernatants accelerated amyloid deposition.
- AEF was purified and found to be approximately 15 KDa.
- AEF is a glycoprotein containing glucose, mannose, galactosamine, and sialic acid.
- AEF was localized in the cytosol of neutrophils from amyloid-laden mice.
Conclusions:
- AEF is a neutrophil-associated glycoprotein that enhances amyloid deposition.
- These findings provide insights into the pathogenesis of amyloidosis.