Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nickel chelating functionalization of graphene composite for metal affinity membrane isolation of lysozyme.

Journal of materials chemistry. B·2020

Same author

In situ growth of β-FeOOH nanorods on graphene oxide with ultra-high relaxivity for in vivo magnetic resonance imaging and cancer therapy.

Journal of materials chemistry. B·2020

Same author

A three-dimensional amylopectin-reduced graphene oxide framework for efficient adsorption and removal of hemoglobin.

Journal of materials chemistry. B·2020

Same author

A novel organic-inorganic hybrid polyoxometalate for the selective adsorption/isolation of β-lactoglobulin.

Journal of materials chemistry. B·2020

Same author

The regulation of hydrophilicity and hydrophobicity of carbon dots via a one-pot approach.

Journal of materials chemistry. B·2020

Same author

Preparation of a cobalt mono-substituted silicotungstic acid doped with aniline for the selective adsorption of ovalbumin.

Journal of materials chemistry. B·2020

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

The isolation of basic proteins by solid-phase extraction with multiwalled carbon nanotubes.

Zhuo Du¹, Yong-Liang Yu, Xu-Wei Chen

¹Research Center for Analytical Sciences, Box 332, Northeastern University, Shenyang 110004, China.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|September 14, 2007

Summary

Multiwalled carbon nanotubes (MWCNTs) offer a novel solid-phase extraction method for isolating basic proteins like hemoglobin and cytochrome c. This technique enables efficient separation and recovery of target proteins from biological samples.

More Related Videos

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

A Protocol for the Identification of Protein-protein Interactions Based on ¹⁵N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Related Experiment Videos

Last Updated: Jul 11, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

A Protocol for the Identification of Protein-protein Interactions Based on ¹⁵N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Area of Science:

Analytical Chemistry
Biochemistry
Materials Science

Background:

Solid-phase extraction (SPE) is crucial for isolating specific proteins from complex biological matrices.
Developing efficient and selective sorbent materials is essential for improving protein purification techniques.

Purpose of the Study:

To investigate the use of multiwalled carbon nanotubes (MWCNTs) as sorbents for the solid-phase extraction of basic proteins.
To develop an online sequential injection system for the selective isolation and separation of hemoglobin and cytochrome c.

Main Methods:

Utilized MWCNTs packed in a microcolumn within a sequential injection system for online protein sorption.
Employed sequential elution with a phosphate buffer (pH 8.0) for hemoglobin and a NaCl solution for cytochrome c.
Analyzed human whole blood samples for hemoglobin isolation and validated results using SDS-PAGE.

Main Results:

Achieved efficient selective sorption and separation of hemoglobin and cytochrome c using MWCNTs.
Obtained high retention efficiencies (100%) and recovery rates (98% for hemoglobin, 90% for cytochrome c).
Demonstrated enrichment factors of 11 for hemoglobin and 15 for cytochrome c with a sampling frequency of 8 h(-1).

Conclusions:

MWCNTs are effective sorbents for the selective solid-phase extraction of basic proteins from biological samples.
The developed sequential injection system provides a rapid and efficient method for protein isolation and separation.
This approach shows practical applicability for analyzing proteins in complex matrices like human whole blood.