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[Effect of microbial immunomodulators on the course of lymphocytic choriomeningitis virus infection in old mice with
1Semmelweis Orvostudományi Egyetem, Mikrobiológiai Intézet, Budapest.
Abstract:
Aged mice with physiological thymus involution were treated intraperitoneally with 0.2 ml of live Newcastle Disease Virus (NDV) containing attenuated NDV vaccine (HA titre: log 2(16)/ml) one day before or with Mannozym (0.1% zymosan suspension) four days and one day before intracerebral inoculation with 100 LD50 dose of LCMV. One dose of M was equal to 40 mg/kg zymosan. Both, NDV vaccine and M pretreatments contributed to the development of fatal lymphocytic choriomeningitis, thus enhanced the cellular immune response to LCMV infection. Present results are compared with earlier results which were attained on young adult suckling mice in similar system with pretreatment with different microbial immunomodulators. Present results reinforced the authors' earlier observations that the direction and degree of immunomodulatory effects can be influenced by the actual conditions, like age, of the immune system.
Insights
Pretreating aged mice with Newcastle Disease Virus (NDV) vaccine or Mannozym (M) enhanced their immune response to lymphocytic choriomeningitis virus (LCMV) infection. Age significantly influences immunomodulatory treatment effects.
Area of Science:
- Immunology
- Virology
- Gerontology
Context:
- Physiological thymus involution in aged mice impacts immune function.
- Newcastle Disease Virus (NDV) vaccine and Mannozym (M) are microbial immunomodulators.
- Lymphocytic choriomeningitis virus (LCMV) infection is used to study cellular immune response.
Purpose:
- To investigate the immunomodulatory effects of NDV vaccine and Mannozym in aged mice.
- To compare the immune response in aged mice with previous studies on young mice.
- To determine how age influences the effectiveness of immunomodulatory treatments.
Summary:
- Aged mice were pretreated with either NDV vaccine or Mannozym before LCMV inoculation.
- Both NDV vaccine and Mannozym pretreatments exacerbated fatal lymphocytic choriomeningitis.
- This enhancement of disease indicated a heightened cellular immune response to LCMV infection.
Impact:
- The study highlights that immunomodulatory effects are age-dependent.
- Results suggest that age is a critical factor in designing immune-boosting strategies.
- Findings contribute to understanding age-related changes in immune system response to viral infections and treatments.