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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Related Experiment Video

Updated: Jul 11, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Atomic force microscopy and spectroscopy of native membrane proteins.

Daniel J Müller1, Andreas Engel

  • 1Center of Biotechnology, University of Technology, Tatzberg 47-51, Dresden, Germany.

Nature Protocols
|September 15, 2007
PubMed
Summary

Atomic force microscopy (AFM) allows detailed structural analysis of membrane proteins in their native buffer environment. This technique reveals molecular interactions by imaging and unfolding single proteins with high resolution.

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Last Updated: Jul 11, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
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Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Membrane proteins constitute 30% of higher organism proteomes.
  • They are crucial for cellular functions like energy conversion and transport.
  • Native environment assessment is key for understanding membrane protein structure and function.

Purpose of the Study:

  • To highlight the utility of Atomic Force Microscopy (AFM) for membrane protein analysis.
  • To demonstrate the capability of AFM in studying proteins within their native environment.
  • To showcase AFM's potential in revealing molecular interactions.

Main Methods:

  • Utilizing Atomic Force Microscopy (AFM) for high-resolution imaging of membrane proteins.
  • Assessing membrane protein structure and surface topography in physiological buffer solutions.
  • Employing AFM to unfold single proteins and investigate molecular interactions.

Main Results:

  • Achieved lateral resolution below 1 nm and vertical resolution of 0.1-0.2 nm for single membrane proteins.
  • Demonstrated direct manipulation and unfolding of individual proteins using AFM.
  • Provided insights into the molecular interactions governing protein structure and function.

Conclusions:

  • AFM is a powerful tool for the structural analysis of membrane proteins in their native environment.
  • The technique allows for high-resolution imaging and mechanical probing of single proteins.
  • AFM facilitates the understanding of molecular interactions critical to membrane protein function.