Related Experiment Video
Updated: Jul 11, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
Glucosinolate concentration in turnip (Brassica rapa ssp. rapifera L.) roots as affected by nitrogen and sulfur
Shumin Li1, Ilona Schonhof, Angelika Krumbein
1Resource and Environmental College, Northeast Agricultural University, Harbin, China. lishumin113@yahoo.com.cn
Abstract:
Three greenhouse pot experiments were conducted with four different nitrogen (N) treatments (80, 160, 240, and 320 kg ha (-1)) in combination with three sulfur (S) treatments (10, 20, and 60 kg ha (-1)) to study the effects of combined N and S supply on glucosinolate concentration and composition in turnip roots. Total glucosinolate concentration varied widely from 9.7 (N 320S 10) to 91.6 (N 160S 60) mg (100 g) (-1) root fresh weight (FW) and individual glucosinolate concentrations were increased with increasing S supply regardless of the N treatment, whereas enhanced N supply (160 - 320 N ha (-1)) at the high S level (60 kg ha (-1)) did not affect total glucosinolate concentration. In contrast, assumingly attributed to the individual glucosinolate biosynthesis concentration of N-containing tryptophan-derived indole glucosinolate was highest with increased N supply, whereas S-containing methionine-derived aromatic and aliphatic glucosinolates decreased with increasing N supply combined at low S level (10-20 kg ha (-1)).
More Related Videos
Related Concept Videos
Key Elements for Plant Nutrition
Sulfur Assimilation
Inorganic Nitrogen Assimilation
The Roles of Bacteria and Fungi in Plant Nutrition
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

